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Pancreatic Neoplasms: HELP
Articles by Ling Liu
Based on 5 articles published since 2010
(Why 5 articles?)
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Between 2010 and 2020, Ling Liu wrote the following 5 articles about Pancreatic Neoplasms.
 
+ Citations + Abstracts
1 Article A novel chemoradiation targeting stem and nonstem pancreatic cancer cells by repurposing disulfiram. 2017

Cong, Juan / Wang, Yangyang / Zhang, Xiao / Zhang, Nan / Liu, Ling / Soukup, Klara / Michelakos, Theodoros / Hong, Theodore / DeLeo, Albert / Cai, Lei / Sabbatino, Francesco / Ferrone, Soldano / Lee, Hang / Levina, Vera / Fuchs, Bryan / Tanabe, Kenneth / Lillemoe, Keith / Ferrone, Cristina / Wang, Xinhui. ·Division of Surgical Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. · Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. · University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA; Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA. · Division of Surgical Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. · Department of Biostatistics Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. · University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA. · Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. · Division of Surgical Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: xwang30@mgh.harvard.edu. ·Cancer Lett · Pubmed #28864067.

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) has a 5-year relative survival rate of 8% and is projected to be the second leading cause of cancer death by 2030, underscoring the urgency to develop new strategies to improve current therapeutic modalities for PDAC. Targeting pancreatic cancer stem cells (PCSCs), which are resistant to radiation and chemotherapy, is a promising strategy. A novel approach which can be readily clinically translated is to repurpose disulfiram (DSF), a drug for treating alcoholism, to target PCSCs. Chemoradiation or the combination of chemotherapy agents FOLFIRINOX, currently standard care for PDAC, can increase stemness in some established or primary PDAC cell lines. However, DSF in the presence of exogenously or endogenously supplied copper (Cu), when combined with chemotherapy or chemoradiation, targets both PCSCs and nonstem PDAC cells. Previously, we demonstrated that DSF/Cu effectively targets breast cancer stem cells in the context of fractionated radiation (FIR) by inhibiting the NF-κB-stemness gene pathway. Therefore, the hypothesis that PCSCs can be effectively targeted by incorporating DSF/Cu into the standard chemoradiation regimen consisting of 5-FU and FIR was investigated and found to be effective in vitro in targeting PCSCs, identified as either ALDH

2 Article Decreased circadian component Bmal1 predicts tumor progression and poor prognosis in human pancreatic ductal adenocarcinoma. 2016

Li, Wenhua / Liu, Ling / Liu, Di / Jin, Shimao / Yang, Yisha / Tang, Wei / Gong, Lei. ·Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: lwh42558@sina.com. · Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: linger3307@126.com. · Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: luddy8891@sina.com. · Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: jinshimao85@sina.com. · Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: yangyisha0503@126.com. · Department of Pathology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: 13771067011@163.com. · Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, No 68, Zhong Shan Road, Wuxi, 214002, China. Electronic address: gonglei0520@yahoo.com.cn. ·Biochem Biophys Res Commun · Pubmed #26915801.

ABSTRACT: The circadian clock has been demonstrated playing important roles in human tumorigenic process; however, the detailed clinical implications of circadian disruption on tumors have not been well understood. In this study, we investigated the expression pattern of Bmal1, the core component of the circadian system, in human pancreatic ductal adenocarcinoma (PDA). Our immunohistochemistry analysis showed that the protein level of Bmal1 was significantly decreased in tumor tissues from 87 patients with PDA compared with adjacent non-cancerous tissues. Low Bmal1 expression was associated with the TNM/clinical stage, histological differentiation, and vascular invasion of PDA; but no significant relevance to patient age, gender, the tumor location, or the size. Furthermore, Kaplan-Meier survival analysis revealed that PDA patients with low Bmal1 expression had shorter overall survival (OS) times as well as disease-free times (DFS) compared to the patients with high Bmal1 expression. Lastly, univariate and multivariate analyses identified low Bmal1 expression as an independent prognostic factor for poor survival outcome for patients with PDA. Collectively, our present study demonstrated that the decreased expression of Bmal1 is correlated with the tumor progression and poor prognosis in human PDA, which implicated its potential to be used as a biomarker for diagnosis and prognosis of PDA.

3 Article The pseudokinase SgK223 promotes invasion of pancreatic ductal epithelial cells through JAK1/Stat3 signaling. 2015

Tactacan, Carole M / Phua, Yu Wei / Liu, Ling / Zhang, Luxi / Humphrey, Emily S / Cowley, Mark / Pinese, Mark / Biankin, Andrew V / Daly, Roger J. ·Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. carole.tactacan@gmail.com. · Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, 3800, Australia. carole.tactacan@gmail.com. · Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, 3800, Australia. yuwei.phua@monash.edu. · Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, 3800, Australia. ling.liu@monash.edu. · Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. luxi.zhang@monash.edu. · Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, 3800, Australia. luxi.zhang@monash.edu. · Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. ehumphrey@biochem.mpg.de. · Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. m.cowley@garvan.org.au. · Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. m.pinese@garvan.org.au. · Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. andrew.biankin@glasgow.ac.uk. · Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Scotland, G61 1BD, UK. andrew.biankin@glasgow.ac.uk. · Cancer Research Division, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia. roger.daly@monash.edu. · Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, 3800, Australia. roger.daly@monash.edu. · Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Level 1, Building 77, 23 Innovation Walk, Monash, VIC, 3800, Australia. roger.daly@monash.edu. ·Mol Cancer · Pubmed #26215634.

ABSTRACT: BACKGROUND: Characterization of molecular mechanisms underpinning development of pancreatic ductal adenocarcinoma (PDAC) may lead to the identification of novel therapeutic targets and biomarkers. SgK223, also known as Pragmin, is a pseudokinase and scaffolding protein closely related to SgK269/PEAK1. Both proteins are implicated in oncogenic tyrosine kinase signaling, but their mechanisms and function remain poorly characterized. METHODS: Expression of SgK223 in PDAC and PDAC cell lines was characterized using gene expression microarrays, mass spectrometry (MS)-based phosphoproteomics and Western blotting. SgK223 was overexpressed in human pancreatic ductal epithelial (HPDE) cells via retroviral transduction, and knocked down in PDAC cells using siRNA. Cell proliferation was determined using a colorimetric cell viability assay, and cell migration and invasion using transwells. Expression of markers of epithelial-mesenchyme transition (EMT) was assayed by quantitative PCR. SgK223 and Stat3 signaling was interrogated by immunoprecipitation, Western blot and gene reporter assays. The functional role of specific kinases and Stat3 was determined using selective small molecule inhibitors. RESULTS: Elevated site-selective tyrosine phosphorylation of SgK223 was identified in subsets of PDAC cell lines, and increased expression of SgK223 detected in several PDAC cell lines compared to human pancreatic ductal epithelial (HPDE) cells and in PDACs compared to normal pancreas. Expression of SgK223 in HPDE cells at levels comparable to those in PDAC did not alter cell proliferation but led to a more elongated morphology, enhanced migration and invasion and induced gene expression changes characteristic of a partial EMT. While SgK223 overexpression did not affect activation of Erk or Akt, it led to increased Stat3 Tyr705 phosphorylation and Stat3 transcriptional activity, and SgK223 and Stat3 associated in vivo. SgK223-overexpressing cells exhibited increased JAK1 activation, and use of selective inhibitors determined that the increased Stat3 signaling driven by SgK223 was JAK-dependent. Pharmacological inhibition of Stat3 revealed that Stat3 activation was required for the enhanced motility and invasion of SgK223-overexpressing cells. CONCLUSIONS: Increased expression of SgK223 occurs in PDAC, and overexpression of SgK223 in pancreatic ductal epithelial cells promotes acquisition of a migratory and invasive phenotype through enhanced JAK1/Stat3 signaling. This represents the first association of SgK223 with a particular human cancer, and links SgK223 with a major signaling pathway strongly implicated in PDAC progression.

4 Article Pancreas Metastasization of Nasal NK/T-Cell Lymphoma: A Case Report. 2015

Zhou, Chong / Bu, Xiangzhao / Li, Xiangyang / Liu, Ling / Xie, Xiaomei / Zhuo, Shichao / Yin, Haitao. ·Department of Radiation Oncology, The Central Hospital of Xuzhou, Liberation, No. 199 South Rd, Xuzhou, 221009, Jiang Su, China. · Department of Pathology, The Central Hospital of Xuzhou, Xuzhou, 221009, China. · Department of Radiation Oncology, The Central Hospital of Xuzhou, Liberation, No. 199 South Rd, Xuzhou, 221009, Jiang Su, China. contribution069@163.com. ·Cell Biochem Biophys · Pubmed #25638348.

ABSTRACT: We report here a case of nasal natural killer/T-cell lymphoma with metastasization into the pancreas. A 43-year-old Chinese female patient presented with right nasal obstruction and purulent rhinorrhea that lasted for the past half year and worsened gradually despite antibiotic medical treatment. Physical examination revealed a mass in the right nasal cavity and an enlarged lymph node in the right submandibular region. A PET/CT scan and immunostaining of the resectate led to a diagnosis of nasal natural killer/T-cell lymphoma, clinical stage IIE, and the International Prognostic Index score of 1. The patient received radiotherapy followed by four cycles of the CHOP chemotherapy. A complete remission was achieved, and the patient was disease-free for 9 months. On a subsequent follow-up, abdominal ultrasound scan, enhanced abdominal CT scan, and ultrasound-guided fine-needle aspiration biopsy revealed pancreatic metastasis secondary to nasal lymphoma. Radiotherapy (40 Gy) to the whole pancreas was done, followed by two courses of the SMILE regime chemotherapy, leading to a complete remission. At the moment, the patient is undergoing subsequent chemotherapy.

5 Article Involvement of the mitochondrial pathway in bruceine D-induced apoptosis in Capan-2 human pancreatic adenocarcinoma cells. 2012

Liu, Ling / Lin, Zhi-Xiu / Leung, Po Sing / Chen, Li-Hua / Zhao, Ming / Liang, Juan. ·School of Chinese Medicine, Faculty of Science, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P.R. China. ·Int J Mol Med · Pubmed #22552257.

ABSTRACT: The fruit of Brucea javanica L. is a common herb used in Chinese medicine for the treatment of a variety of cancers. Our research group has previously identified bruceine D (BD), a quassinoid found abundantly in B. javanica, to have potent cytotoxic effect on a number of pancreatic cancer cell lines, including Panc-1, SW1990 and Capan-1 cells. In the present study, we showed that BD was also able to inhibit the growth of the Capan-2 human pancreatic adenocarcinoma cell line, but it exerted only modest cytotoxicity on the WRL68 human hepatocyte cell line and a human pancreatic progenitor cell line. The antiproliferative effects of BD were comparable to those exhibited by camptothecin and gemcitabine in our culture system. We found a dose-dependent decrease of the mitochondrial membrane potential in BD-treated Capan-2 cells as measured by the JC-1 assay. BD exposure was able to attenuate the expression of Bcl-2 protein in Capan-2 cells as detected by western blot analysis. In addition, the expression of both caspase 9 and caspase 3 in BD-treated Capan-2 cells was significantly accentuated. Moreover, BD was capable of inducing the fragmentation of genomic DNA in Capan-2 cells as evidenced by Hoechst staining. Cell cycle analysis demonstrated that BD could increase the percentage of Capan-2 cells in the subG1 phase in a dose-related manner. An increase in the apoptosis of Capan-2 cells was also observed by Annexin V and PI staining. These results unequivocally indicate that BD induces cytotoxicity in Capan-2 cells via the induction of cellular apoptosis involving the mitochondrial pathway.