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Pancreatic Neoplasms: HELP
Articles by Michael J. Goldberg
Based on 5 articles published since 2009
(Why 5 articles?)
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Between 2009 and 2019, Michal Goldberg wrote the following 5 articles about Pancreatic Neoplasms.
 
+ Citations + Abstracts
1 Article Tumor cells derived exosomes contain hTERT mRNA and transform nonmalignant fibroblasts into telomerase positive cells. 2016

Gutkin, Anna / Uziel, Orit / Beery, Einat / Nordenberg, Jardena / Pinchasi, Maria / Goldvaser, Hadar / Henick, Steven / Goldberg, Michal / Lahav, Meir. ·The Felsenstein Medical Research Center, Rabin Medical Center, Petah Tikva, Israel. · Sackler School of Medicine, Tel-Aviv University, Petah Tikva, Israel. · Institute of Oncology, Davidoff Cancer Center, Rabin Medical Center, Petah Tikva, Israel. · Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Petah Tikva, Israel. · Institute of Hematology, Davidoff Cancer Center, Rabin Medical Center, Petah Tikva, Israel. ·Oncotarget · Pubmed #27385095.

ABSTRACT: Exosomes are small (30-100nm) vesicles secreted from all cell types serving as inter-cell communicators and affecting biological processes in "recipient" cells upon their uptake. The current study demonstrates for the first time that hTERT mRNA, the transcript of the enzyme telomerase, is shuttled from cancer cells via exosomes into telomerase negative fibroblasts, where it is translated into a fully active enzyme and transforms these cells into telomerase positive, thus creating a novel type of cells; non malignant cells with telomerase activity. All tested telomerase positive cells, including cancer cells and non malignant cells with overexpressed telomerase secreted exosomal hTERT mRNA in accordance with the endogenous levels of their hTERT mRNA and telomerase activity. Similarly exosomes isolated from sera of patients with pancreatic and lung cancer contained hTERT mRNA as well. Telomerase activity induced phenotypic changes in the recipient fibroblasts including increased proliferation, extension of life span and postponement of senescence. In addition, telomerase activity protected the fibroblasts from DNA damage induced by phleomycin and from apoptosis, indicating that also telomerase "extracurricular" activities are manifested in the recipient cells. The shuttle of telomerase from cancer cells into fibroblasts and the induction of these changes may contribute to the alterations of cancer microenvironment and its role in cancer. The described process has an obvious therapeutic potential which will be explored in further studies.

2 Article In vivo risk analysis of pancreatic cancer through optical characterization of duodenal mucosa. 2015

Mutyal, Nikhil N / Radosevich, Andrew J / Bajaj, Shailesh / Konda, Vani / Siddiqui, Uzma D / Waxman, Irving / Goldberg, Michael J / Rogers, Jeremy D / Gould, Bradley / Eshein, Adam / Upadhye, Sudeep / Koons, Ann / Gonzalez-Haba Ruiz, Mariano / Roy, Hemant K / Backman, Vadim. ·From the *Department of Biomedical Engineering, Northwestern University; †Department of Internal Medicine, NorthShore University HealthSystems, Evanston; ‡Center for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL; and §Department of Gastroenterology, Boston Medical Center, Boston, MA. ·Pancreas · Pubmed #25906443.

ABSTRACT: OBJECTIVES: To reduce pancreatic cancer mortality, a paradigm shift in cancer screening is needed. Our group pioneered the use of low-coherence enhanced backscattering (LEBS) spectroscopy to predict the presence of pancreatic cancer by interrogating the duodenal mucosa. A previous ex vivo study (n = 203) demonstrated excellent diagnostic potential: sensitivity, 95%; specificity, 71%; and accuracy, 85%. The objective of the current case-control study was to evaluate this approach in vivo. METHODS: We developed a novel endoscope-compatible fiber-optic probe to measure LEBS in the periampullary duodenum of 41 patients undergoing upper endoscopy. This approach enables minimally invasive detection of the ultrastructural consequences of pancreatic field carcinogenesis. RESULTS: The LEBS parameters and optical properties were significantly altered in patients harboring adenocarcinomas (including early-stage) throughout the pancreas relative to healthy controls. Test performance characteristics were excellent with sensitivity = 78%, specificity = 85%, and accuracy = 81%. Moreover, the LEBS prediction rule was not confounded by patients' demographics. CONCLUSION: We demonstrate the feasibility of in vivo measurement of histologically normal duodenal mucosa to predict the presence of adenocarcinoma throughout the pancreas. This represents the next step in establishing duodenal LEBS analysis as a prescreening technique that identifies clinically asymptomatic patients who are at elevated risk of PC.

3 Article Spatially resolved optical and ultrastructural properties of colorectal and pancreatic field carcinogenesis observed by inverse spectroscopic optical coherence tomography. 2014

Yi, Ji / Radosevich, Andrew J / Stypula-Cyrus, Yolanda / Mutyal, Nikhil N / Azarin, Samira Michelle / Horcher, Elizabeth / Goldberg, Michael J / Bianchi, Laura K / Bajaj, Shailesh / Roy, Hemant K / Backman, Vadim. ·Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208. · NorthShore University Health Systems, Department of Internal Medicine, Evanston, Illinois 60201. · Boston Medical Center, Department of Medicine, Boston, Massachusetts 02118. ·J Biomed Opt · Pubmed #24643530.

ABSTRACT: Field carcinogenesis is the initial stage of cancer progression. Understanding field carcinogenesis is valuable for both cancer biology and clinical medicine. Here, we used inverse spectroscopic optical coherence tomography to study colorectal cancer (CRC) and pancreatic cancer (PC) field carcinogenesis. Depth-resolved optical and ultrastructural properties of the mucosa were quantified from histologically normal rectal biopsies from patients with and without colon adenomas (n=85) as well as from histologically normal peri-ampullary duodenal biopsies from patients with and without PC (n=22). Changes in the epithelium and stroma in CRC field carcinogenesis were separately quantified. In both compartments, optical and ultra-structural alterations were consistent. Optical alterations included lower backscattering (μb) and reduced scattering (μs') coefficients and higher anisotropy factor g. Ultrastructurally pronounced alterations were observed at length scales up to ∼450  nm, with the shape of the mass density correlation function having a higher shape factor D, thus implying a shift to larger length scales. Similar alterations were found in the PC field carcinogenesis despite the difference in genetic pathways and etiologies. We further verified that the chromatin clumping in epithelial cells and collagen cross-linking caused D to increase in vitro and could be among the mechanisms responsible for the observed changes in epithelium and stroma, respectively.

4 Article Ultrastructural alterations in field carcinogenesis measured by enhanced backscattering spectroscopy. 2013

Radosevich, Andrew J / Mutyal, Nikhil N / Yi, Ji / Stypula-Cyrus, Yolanda / Rogers, Jeremy D / Goldberg, Michael J / Bianchi, Laura K / Bajaj, Shailesh / Roy, Hemant K / Backman, Vadim. ·Northwestern University, Department of Biomedical Engineering, Tech E310, 2145 Sheridan Road, Evanston, Illinois 60208. ·J Biomed Opt · Pubmed #24008865.

ABSTRACT: Optical characterization of biological tissue in field carcinogenesis offers a method with which to study the mechanisms behind early cancer development and the potential to perform clinical diagnosis. Previously, low-coherence enhanced backscattering spectroscopy (LEBS) has demonstrated the ability to discriminate between normal and diseased organs based on measurements of histologically normal-appearing tissue in the field of colorectal (CRC) and pancreatic (PC) cancers. Here, we implement the more comprehensive enhanced backscattering (EBS) spectroscopy to better understand the structural and optical changes which lead to the previous findings. EBS provides high-resolution measurement of the spatial reflectance profile P(rs) between 30 microns and 2.7 mm, where information about nanoscale mass density fluctuations in the mucosa can be quantified. A demonstration of the length-scales at which P(rs) is optimally altered in CRC and PC field carcinogenesis is given and subsequently these changes are related to the tissue's structural composition. Three main conclusions are made. First, the most significant changes in P(rs) occur at short length-scales corresponding to the superficial mucosal layer. Second, these changes are predominantly attributable to a reduction in the presence of subdiffractional structures. Third, similar trends are seen for both cancer types, suggesting a common progression of structural alterations in each.

5 Article Serum biomarker panels for the detection of pancreatic cancer. 2011

Brand, Randall E / Nolen, Brian M / Zeh, Herbert J / Allen, Peter J / Eloubeidi, Mohamad A / Goldberg, Michael / Elton, Eric / Arnoletti, Juan P / Christein, John D / Vickers, Selwyn M / Langmead, Christopher J / Landsittel, Douglas P / Whitcomb, David C / Grizzle, William E / Lokshin, Anna E. ·Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA. ·Clin Cancer Res · Pubmed #21325298.

ABSTRACT: PURPOSE: Serum-biomarker based screening for pancreatic cancer could greatly improve survival in appropriately targeted high-risk populations. EXPERIMENTAL DESIGN: Eighty-three circulating proteins were analyzed in sera of patients diagnosed with pancreatic ductal adenocarcinoma (PDAC, n = 333), benign pancreatic conditions (n = 144), and healthy control individuals (n = 227). Samples from each group were split randomly into training and blinded validation sets prior to analysis. A Metropolis algorithm with Monte Carlo simulation (MMC) was used to identify discriminatory biomarker panels in the training set. Identified panels were evaluated in the validation set and in patients diagnosed with colon (n = 33), lung (n = 62), and breast (n = 108) cancers. RESULTS: Several robust profiles of protein alterations were present in sera of PDAC patients compared to the Healthy and Benign groups. In the training set (n = 160 PDAC, 74 Benign, 107 Healthy), the panel of CA 19-9, ICAM-1, and OPG discriminated PDAC patients from Healthy controls with a sensitivity/specificity (SN/SP) of 88/90%, while the panel of CA 19-9, CEA, and TIMP-1 discriminated PDAC patients from Benign subjects with an SN/SP of 76/90%. In an independent validation set (n = 173 PDAC, 70 Benign, 120 Healthy), the panel of CA 19-9, ICAM-1 and OPG demonstrated an SN/SP of 78/94% while the panel of CA19-9, CEA, and TIMP-1 demonstrated an SN/SP of 71/89%. The CA19-9, ICAM-1, OPG panel is selective for PDAC and does not recognize breast (SP = 100%), lung (SP = 97%), or colon (SP = 97%) cancer. CONCLUSIONS: The PDAC-specific biomarker panels identified in this investigation warrant additional clinical validation to determine their role in screening targeted high-risk populations.