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Hearing Disorders: HELP
Articles by Suo-qiang Zhai
Based on 17 articles published since 2010
(Why 17 articles?)
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Between 2010 and 2020, S-Q Zhai wrote the following 17 articles about Hearing Disorders.
 
+ Citations + Abstracts
1 Review Review of experimental and clinical studies of autoimmune sensorineural hearing loss. 2015

Zhai, S Q. ·Institute of Otolaryngology, General Hospital of People's Liberation Army, Beijin, China - zhaisq@plagh.com.cn. ·Minerva Med · Pubmed #25532537.

ABSTRACT: The purpose of this review was to analyze recent studies, both clinical and experimental, on diagnosis and treatment of autoimmune sensorineural hearing loss (ASNHL). Relevant studies were identified by searching Chinese Biomedical Literature Database and China Academic Journals Full-text Database for articles published nationally during the period of 1993-2013. The manuscripts were analyzed for the following aspects: selection of patients, sample size, presence of control group, experimental methods, study outcomes including audiological evaluation results, and efficacy of treatment. Forty-one manuscripts were identified including 12 reviews, 2 case reports, 20 experimental studies, and 7 clinical studies. The patient numbers in clinical studies ranged from 14 to 71 patients. Five clinical studies included control group of healthy individuals, while further two studies had no control group. Patients with ASNHL were treated with corticosteroids (prednisone or dexamethasone) in all seven clinical studies. However, statistical analyses of therapeutic efficacy were not carried out. Animal models were developed by immunization with allogeneic inner ear antigen, and some pathological changes were not specific to the disease. Experimental animals also had various degrees of changes in auditory brainstem response. Treatment guidelines need to be developed, while specific criteria of treatment efficacy need to be established. Further research is needed to demonstrate that experimental ASNHL resembles pathophysiologically the clinical situation.

2 Article Acid stimulation-induced semi-pluripotent characteristics in human somatic cells. 2019

Xu, Qing-Qing / Guo, Wei-Wei / Wang, Xiangdong / Zhai, Suoqiang / Yang, Shiming. ·a Department of Allergy , Beijing Tongren Hospital , Beijing , China. · b Department of Otolaryngology, Head & Neck Surgery, Institute of Otolaryngology , Chinese PLA General Hospital , Beijing , China. · c Department of ENT , Beijing Tongren Hospital , Beijing , China. · d PLA General Hospital, Institute of Otorhinolaryngology , Beijing , China. · e Department of Otolaryngology, Head and Neck Surgery , Chinese PLA General Hospital , Beijing , China. ·Acta Otolaryngol · Pubmed #30734622.

ABSTRACT: BACKGROUND: Clinical trials of cell-based therapies using induced pluripotent stem (iPS) cells have already been started for several neurological diseases. OBJECTIVE: The purpose of the present study was to explore the characteristics and differentiation of somatic cells in vitro undergoing a low pH treatment, so as to provide new therapeutic strategies for treating sensorineural hearing loss. METHODS: Somatic cells were treated with low pH solution to alter their characteristics. In addition, a mouse model of the cochlear lesion was constructed using bilirubin. Subsequently, the characteristics and therapeutic effect of somatic cells undergoing low pH treatment were examined by morphology, alkaline phosphatase (AKP) activity, immunofluorescence assay and q-PCR. RESULTS: The cells in the experimental group grew better than those in the control group. The AKP activity in the experimental group was higher than that in the control group. The expression of Nanog and Oct4 was both positive in the two groups. When the cells were changed to neurobasal medium, the marker of nestin was positive. CONCLUSION: The human somatic cells undergoing a low pH treatment showed the similar characteristics as those of iPS cells, although the functions and therapeutic effect of these altered human somatic cells need to be further studied.

3 Article Molecular mechanisms underlying the protective effects of hydrogen-saturated saline on noise-induced hearing loss. 2017

Chen, Liwei / Han, Mingkun / Lu, Yan / Chen, Daishi / Sun, Xuejun / Yang, Shiming / Sun, Wei / Yu, Ning / Zhai, Suoqiang. ·a Department of Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , China. · b Department of Otolaryngology Head and Neck Surgery , Affiliated Fuzhou First Hospital of Fujian Medical University , Fujian , China. · c Department of Neurosurgery Erlangen , Medical Faculty of the Friedrich Alexander University of Erlangen-Numberg , Erlangen , Germany. · d Department of Diving Medicine, Faculty of Naval Medicine , Second Military Medical University , Shanghai , China. · e Department of Communicative Disorders and Sciences , University at Buffalo The State University of New York , Buffalo , NY , USA. ·Acta Otolaryngol · Pubmed #28549396.

ABSTRACT: OBJECTIVES: This study aimed to explore the molecular mechanism of the protective effects of hydrogen-saturated saline on NIHL. METHODS: Guinea pigs were divided into three groups: hydrogen-saturated saline; normal saline; and control. For saline administration, the guinea pigs were given daily abdominal injections 3 d before and 1 h before noise exposure. ABR were tested to examine cochlear physiology changes. The changes of 8-hydroxy-desoxyguanosine (8-HOdG), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), intercellular cell adhesion molecule-1 (ICAM-1) and high mobility group box-1 protein (HMGB1) in the cochlea were also examined. RESULTS: The results showed that pre-treatment with hydrogen-saturated saline could significantly attenuate noise-induced hearing loss. The concentration of 8-HOdG was also significantly decreased in the hydrogen-saturated saline group compared with the normal saline group. After noise exposure, the concentrations of IL-1, IL-6, TNF-α, and ICAM-1 in the cochlea of guinea pigs in the hydrogen-saturated saline group were dramatically reduced compared to those in the normal saline group. The concentrations of HMGB-1 and IL-10 in the hydrogen-saturated saline group were significantly higher than in those in the normal saline group immediately and at 7 d after noise exposure. CONCLUSIONS: This study revealed for the first time the protective effects of hydrogen-saturated saline on noise-induced hearing loss (NIHL) are related to both the anti-oxidative activity and anti-inflammatory activity.

4 Article A de novo silencer causes elimination of MITF-M expression and profound hearing loss in pigs. 2016

Chen, Lei / Guo, Weiwei / Ren, Lili / Yang, Mingyao / Zhao, Yaofeng / Guo, Zongyi / Yi, Haijin / Li, Mingzhou / Hu, Yiqing / Long, Xi / Sun, Boyuan / Li, Jinxiu / Zhai, Suoqiang / Zhang, Tinghuan / Tian, Shilin / Meng, Qingyong / Yu, Ning / Zhu, Dan / Tang, Guoqing / Tang, Qianzi / Ren, Liming / Liu, Ke / Zhang, Shihua / Che, Tiandong / Yu, Zhengquan / Wu, Nan / Jing, Lan / Zhang, Ran / Cong, Tao / Chen, Siqing / Zhao, Yiqiang / Zhang, Yue / Bai, Xiaoqing / Guo, Ying / Zhao, Lidong / Zhang, Fengming / Zhao, Hui / Zhang, Liang / Hou, Zhaohui / Zhao, Jiugang / Li, Jianan / Zhang, Lijuan / Sun, Wei / Zou, Xiangang / Wang, Tao / Ge, Liangpeng / Liu, Zuohua / Hu, Xiaoxiang / Wang, Jingyong / Yang, Shiming / Li, Ning. ·State Key Laboratory for Agrobiotechnology, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, 100193, China. · Key Laboratory of Pig Industry Sciences (Ministry of Agriculture), Chongqing Academy of Animal Science, Chongqing, 402460, China. · Department of Otolaryngology, Head & Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, 100853, China. · Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China. · Department of Communicative Disorders and Sciences, Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, New York, USA. · Key Laboratory of Pig Industry Sciences (Ministry of Agriculture), Chongqing Academy of Animal Science, Chongqing, 402460, China. kingyou@vip.sina.com. · Department of Otolaryngology, Head & Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, 100853, China. yangsm301@263.net. · State Key Laboratory for Agrobiotechnology, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, 100193, China. ninglcau@cau.edu.cn. ·BMC Biol · Pubmed #27349893.

ABSTRACT: BACKGROUND: Genesis of novel gene regulatory modules is largely responsible for morphological and functional evolution. De novo generation of novel cis-regulatory elements (CREs) is much rarer than genomic events that alter existing CREs such as transposition, promoter switching or co-option. Only one case of de novo generation has been reported to date, in fish and without involvement of phenotype alteration. Yet, this event likely occurs in other animals and helps drive genetic/phenotypic variation. RESULTS: Using a porcine model of spontaneous hearing loss not previously characterized we performed gene mapping and mutation screening to determine the genetic foundation of the phenotype. We identified a mutation in the non-regulatory region of the melanocyte-specific promoter of microphthalmia-associated transcription factor (MITF) gene that generated a novel silencer. The consequent elimination of expression of the MITF-M isoform led to early degeneration of the intermediate cells of the cochlear stria vascularis and profound hearing loss, as well as depigmentation, all of which resemble the typical phenotype of Waardenburg syndrome in humans. The mutation exclusively affected MITF-M and no other isoforms. The essential function of Mitf-m in hearing development was further validated using a knock-out mouse model. CONCLUSIONS: Elimination of the MITF-M isoform alone is sufficient to cause deafness and depigmentation. To our knowledge, this study provides the first evidence of a de novo CRE in mammals that produces a systemic functional effect.

5 Article Clinical efficacy of nerve growth factor in the treatment of blast-induced hearing loss: a pilot study. 2015

Zhai, S-Q / Yu, N / Zhu, Y-H / Chen, L-W / Ma, Y-Y. ·Institute of Otorhinolaryngology, Chinese PLA General Hospital, Beijing, P.R. China. zhaisq@301hospital.com.cn. ·Eur Rev Med Pharmacol Sci · Pubmed #26400515.

ABSTRACT: OBJECTIVE: There is no effective therapy for blast-induced hearing loss in the clinic. The present report summaries our case series with using nerve growth factor in the treatment of patients with various blast-induced hearing loss. PATIENTS AND METHODS: This retrospective study analyzed the clinical outcomes of 21 patients (33 ears) seen in our Outpatient Service Clinic who were treated with nerve growth factor (NGF) by intramuscular injection for 10 days. The pure tone audiometry changes before and after NGF treatments were measured for five frequencies. RESULTS: Among the 21 patients with blast-induced hearing loss (33 ears) treated with 10-d NGF injection, the mean value of pure tone audiometry for the 5 frequencies for all the 33 ears after NGF treatment was significantly improved (p = 0.01). Three patients (6 ears) had hearing improved in different degrees, with average hearing being recovered by 15 dB HL. The total effective rate of the treatment was 18%. CONCLUSIONS: Nerve growth factor can be used to treat blast-induced hearing loss in clinic. The key to clinical success is early treatment; the efficacy on the patients with late treatment (more than one-month after injury is poor).

6 Article [Meta-analysis on effectiveness of prelingually deaf patients at different ages following cochlear implantation]. 2015

Xu, Qingqing / Zhai, Suoqiang / Han, Dongyi / Yang, Shiming / Shen, Weidong. · ·Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi · Pubmed #26121827.

ABSTRACT: OBJECTIVE: To assess the clinical effeetiveness of prelingually deaf children after cochlear implantation at different ages so as to provide reasonable expectations for the patients and guidance for the clinical treatment. METHOD: Electronic databases PubMed, YZ365. COM, WANFANG DATA, CMJD, CHKD, CNKI were searched using relevant keywords. Extracted data included author, year of publication, diagnosis, et al. Reported treatment outcomes were clustered into speech discrimination and hearing abilities. Meta-analyses were performed on studies with numerical results using random or fixed effects model. RESULT: There were eight randomized control studies including 442 patients. Comparing speech perception of prelingually deaf children after cochlear implantation younger than three years old (experimental group) and 3-6 years old (control group), three and six months after operation showed that experimental group performed significantly worse than control group; 12 months after operation showed that experimental group performed significantly better than control group. Comparing hearing abilities, three and six months after operation showed that experimental group performed significantly worse than control group; 12 months after operation showed showed that experimental group performed significantly better than control group. Comparing speech perception of younger or older than 4. 5 years old children showed that after 1.5-2 years of operation children implanted younger than 4.5 years of age performed significantly better than children implanted older than 4.5 years old. Comparing speech perception of 7-12 years old children showed that after 3, 6, 12 months of operation patients of 7-12 years old performed significantly better than those children older than 12 years old. Comparing speech perception of implantation younger or older than 18 years old (7-14 yeas old was group A, > 14-18 yeas old was group B, older than 18 yeas old was group C) showed that after one and four years of operation A > B > C, and there were significant differences among them. Comparing warble tone threshold average (WTA) showed that after one year of operation A < B < C, and there were significant differences among them. However, after four years of operation, there was no significant difference among them. CONCLUSION: Prelinguistically deafened patients younger than three years old with cochlear implantation, insisting on scienctific rehabilitation training for a long period of time can receive the optimal recovery effect. The older patients are suggested as early as possible receiving cochlear implantation. The longer they are implanted, the better results they will receive. Moreover, the younger age they are implanted, the faster postoperative language progress they will receive. Further controlled studies with longer follow-up periods and more person included may make the effectiveness of cochlear implantaion more reliable.

7 Article Clinical observation on hearing conditions of centenarians in northern district of China. 2015

Liu, Chen-Qing / Cheng, Xiao-Ting / Zhu, Yu-Hua / Shen, Wei-Dong / Bian, Bo-Wen / Cao, Ju-Yang / Zhai, Suo-Qiang / Chen, Chuan-Xin / Yang, Shi-Ming / Yuan, Hui-Jun / Yu, Ning. ·Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital , Beijing. ·Acta Otolaryngol · Pubmed #25739867.

ABSTRACT: CONCLUSION: The hearing conditions of the centenarians were quite poor as regards hearing thresholds and speech detection ability. OBJECTIVE: To investigate hearing conditions of centenarians. METHODS: A total of 54 centenarians in Rizhao and Linyi Districts in Shandong Province were investigated to assess hearing conditions of centenerians comprehensively by questionnaire investigation, pure-tone audiometry, acoustic immitance, intelligence evaluation, and speech detection scores. Also, 135 individuals were recruited as controls and divided into four groups according to their age: 45-59 years, 60-69 years, 70-79 years, and 80-89 years. RESULTS: The hearing thresholds of the centenarians were dramatically higher than those of the control group (p < 0.05) and all centenarians suffered moderate to profound hearing loss according to the World Health Organization (WHO) criteria. Few centenarians had normal level of speech detection scores. All centenarians showed descending hearing curve, and the hearing threshold of the male centenarians at 8000 Hz was higher than that of the females (p = 0.047). There was a significant air-bone conduction gap in the centenarians (p < 0.05).

8 Article Mutations in the mitochondrial 12S rRNA gene in elderly Chinese people. 2015

Zhu, Yuhua / Zhao, Jiandong / Feng, Bo / Su, Yu / Kang, Dongyang / Yuan, Huijun / Zhai, Suoqiang / Dai, Pu. ·Department of Otorhinolaryngology, Head and Neck Surgery, PLA General Hospital , Beijing , PR China. ·Acta Otolaryngol · Pubmed #25376778.

ABSTRACT: CONCLUSION: Our data indicate that the mitochondrial 12S rRNA gene, and particularly the A827G mutation, may be associated with susceptibility to age-related hearing loss. OBJECTIVE: Hearing loss associated with aging is common among elderly persons. In all genetic backgrounds, mitochondrial DNA (mtDNA) mutations may be one of the most important factors contributing to aging and age-related hearing loss. The mitochondrial 12S rRNA is a hot spot for deafness-associated mutations in Chinese populations. The purpose of the present study was to elucidate the relationship of 12S rRNA gene polymorphisms and age-related hearing loss. METHODS: The 12S rRNA gene polymorphisms were detected by direct sequencing. Statistical analyses were performed to assess the associations between age-related hearing loss and 12S rRNA gene variants. RESULTS: We report here a systematic mutational screening of the mitochondrial 12S rRNA gene in 662 elderly subjects from the general population with various hearing threshold levels (211 controls and 451 age-related hearing loss subjects). Mutational screening of the mitochondrial 12S rRNA gene identified 55 nucleotide changes, including 4 mutations localized at highly conserved sites and 51 known variants. Of the known deafness-associated mutations in the mitochondrial 12S rRNA gene, the incidence of the A1555G mutation was 0.15%, A827G was 4.38%, T1095C was 0.45%, and T1005C was 3.78%. The incidence of the other known variants was 0.15-99.85%. We found statistically significant differences in the proportions of subjects with the A827G mutation among the various age-related hearing loss groups and normal controls.

9 Article [Meta-analysis of the efficacy of cochlear implantation in deaf patients with inner ear malformation]. 2014

Xu, Qingqing / Zhai, Suoqiang / Han, Dongyi / Yang, Shiming / Shen, Weidong. · ·Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi · Pubmed #26248452.

ABSTRACT: OBJECTIVE: Assess and compare the clinical efficacy and safety of cochlear implantation in deaf patients with inner ear malformation and in the ones with normal inner ear structure, so that to clarify whether it is effective to restore hearing for the deaf patients with inner ear malformation. METHOD: The literature with relevant key words were retrieved in the databases including PubMed, YZ365. com, WANFANG data, CMJD, CHKD and CNKI with language limited to Chinese and English. Extracted data included author, year of publication, diagnosis, treatment, et al. The clinical efficacy of cochlear implantation was assessed by the complications, electrode impedance, behavior T-level, hearing abilities and speech discrimination. Meta-analysis was performed using random or fixed effects model according to the heterogeneity of data. RESULT: There were 11 randomized control studies involving 655 patients included in this study. There was no statistically significant difference among the deaf patients in mixed inner ear-malformation group, Mondini group and large vestibular aqueduct syndrome group in the aspects of postoperative complications, electrode impedance, behavior T-level, hearing abilities and speech discrimination. CONCLUSION: Cochlear implantation could be the way of treatment and rehabilitation for deaf patients with inner ear malformation. Further controlled studies with longer follow-up periods and more multiracial cases included may help to evaluate the efficacy of cochlear implantation for deaf patients with inner ear malformation more reliably.

10 Article Hydrogen-saturated saline protects intensive narrow band noise-induced hearing loss in guinea pigs through an antioxidant effect. 2014

Chen, Liwei / Yu, Ning / Lu, Yan / Wu, Longjun / Chen, Daishi / Guo, Weiwei / Zhao, Lidong / Liu, Mingbo / Yang, Shiming / Sun, Xuejun / Zhai, Suoqiang. ·Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China. · Department of Otolaryngology Head and Neck Surgery, Wenzhou Medical College, Wenzhou, Zhejiang Province, China. · Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China. ·PLoS One · Pubmed #24945316.

ABSTRACT: The purpose of the current study was to evaluate hydrogen-saturated saline protecting intensive narrow band noise-induced hearing loss. Guinea pigs were divided into three groups: hydrogen-saturated saline; normal saline; and control. For saline administration, the guinea pigs were given daily abdominal injections (1 ml/100 g) 3 days before and 1 h before narrow band noise exposure (2.5-3.5 kHz 130 dB SPL, 1 h). The guinea pigs in the control group received no treatment. The hearing function was assessed by the auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) recording. The changes of free radicals in the cochlea before noise exposure, and immediately and 7 days after noise exposure were also examined. By Scanning electron microscopy and succinate dehydrogenase staining, we found that pre-treatment with hydrogen-saturated saline significantly reduced noise-induced hair cell damage and hearing loss. We also found that the malondialdehyde, lipid peroxidation, and hydroxyl levels were significantly lower in the hydrogen-saturated saline group after noise trauma, indicating that hydrogen-saturated saline can decrease the amount of harmful free radicals caused by noise trauma. Our findings suggest that hydrogen-saturated saline is effective in preventing intensive narrow band noise-induced hearing loss through the antioxidant effect.

11 Article Analysis of the heteroplasmy level and transmitted features in hearing-loss pedigrees with mitochondrial 12S rRNA A1555G mutation. 2014

Zhu, Yuhua / Huang, Shasha / Kang, Dongyang / Han, Mingyu / Wang, Guojian / Yuan, Yongyi / Su, Yu / Yuan, Huijun / Zhai, Suoqiang / Dai, Pu. ·Department of Otorhinolaryngology, Head and Neck Surgery, PLA General Hospital, 28# Fuxing Road, Beijing 100853, P, R, China. zhaisq@plagh.com.cn. ·BMC Genet · Pubmed #24533451.

ABSTRACT: BACKGROUND: Mitochondrial cytopathies are characterized by a large variability of clinical phenotypes and severity. The amount of mutant mitochondrial DNA (mtDNA) in a cell, called the heteroplasmy level, is an important determinant of the degree of mitochondrial dysfunction and therefore disease severity. Understanding the distribution of heteroplasmy levels across a group of offspring is an important step in understanding the inheritance of diseases. Recently, the mtDNA A1555G mutation was found to be associated with non-syndromic and drug-induced hearing loss. RESULTS: Here, we report five pedigrees with multiple members having the A1555G mutation and showing diverse clinical manifestations and different heteroplasmy levels. Clinical evaluations revealed that the hearing impairment phenotypes varied with respect to the severity of hearing loss, age of onset of hearing loss, and pattern of audiometric configuration. These five Chinese pedigrees had different penetrance of hearing loss, ranging from 10-52%. A molecular study showed that the average heteroplasmy rates of the five pedigrees were 31.98% (0-91.35%), 78.28% (32.8-96.08%), 87.99% (82.32-94.65%), 93.34% (91.02-95.05%), and 93.57% (91.38-94.24%). There was no gradual tendency of heteroplasmy to increase or decrease along with transmission. A study of the relationship between clinical features and genetic background found that the percentage of deafness was 0 when the heteroplasmy level was less than 50%, 25% when the heteroplasmy level was 50-80%, 47.06% when the heteroplasmy level was 80-90%, and 57.58% when the heteroplasmy level exceeded 90%. The risk of deafness rose with the heteroplasmy level. CONCLUSIONS: The results suggest that there are large random shifts in the heteroplasmy level between mothers and offspring with the A1555G mutation; heteroplasmy could disappear randomly when the heteroplasmy level of the pedigree was low enough, and no regular pattern was found. The heteroplasmy level may be one of the factors influencing the penetrance of deafness caused by the mtDNA A1555G mutation.

12 Article Clinical investigation on the beneficial effects of the Chinese medicinal herb Gushen Pian on sensorineural deafness and tinnitus. 2013

Zhai, Suoqiang / Fang, Yaoyun / Yang, Weiyan / Gu, Rui / Han, Dongyi / Yang, Shiming. ·Institute of Otorhinolaryngology of Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China, zhaisq@plagh.com.cn. ·Cell Biochem Biophys · Pubmed #23516092.

ABSTRACT: The objective is to study the therapeutic effects of Gushen Pian on sensorineural deafness according to the Phase II clinical trial protocol, as approved for novel traditional Chinese medicines by Ministry of Health of PRC. This is a double blind study in which 120 patients were allocated randomly into treatment and control groups and an open treatment group (40 cases in each group). Patients in the treatment groups were administrated with Gushen Pian and controls received placebo. Routine examination of blood and urine, hepatic and renal function tests and pure tone audiometry were performed before and after treatment. Clinical symptoms and therapeutic outcomes were compared and evaluated. For double-blind treatment group, the total effective rate of deafness was 42.2% and total relieved rate of deafness was 4.6%; for double-blind control group, the total effective rate of deafness was 18.7% and total relieved rate of deafness was 0%; for simple treatment group, the total effective rate of deafness was 58.7% and total relieved rate of deafness was 6.3%. For double-blind treatment group, the total effective rate of tinnitus was 89.2% and total relieved rate of tinnitus was 59.5%; for double-blind control group, the total effective rate of tinnitus was 30.8% and total relieved rate of tinnitus was 5.1%; for simple treatment group, the total effective rate of tinnitus was 74.3% and total relieved rate of tinnitus was 57.1%. The double-blind treatment showed statistically significant differences from control group. The medication could effectively alleviate aural fullness, dizziness, lassitude of loins and knees, dysphoria with feverish sensation in chest, insomnia, and fatigue, etc. No adverse effect was reported during treatment; no abnormal results were reported in blood, urine, faces, heart function, liver function and kidney function examination. Gushen Pian had beneficial effect on deafness and tinnitus and could effectively alleviate aural fullness, insomnia, etc., without any adverse effects.

13 Article Mitochondria toxin-induced acute cochlear cell death indicates cellular activity-correlated energy consumption. 2013

Zou, Jing / Zhang, Ya / Zhang, Weikai / Poe, Dennis / Zhai, Suoqiang / Yang, Shiming / Pyykkö, Ilmari. ·Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China. Jing.Zou@uta.fi ·Eur Arch Otorhinolaryngol · Pubmed #23179932.

ABSTRACT: The different cell types within the cochlea may have a specific contribution to the pathological changes during metabolism failure, which may provide clues for developing novel strategies for inner ear therapy. In order to evaluate activity-correlated cell death during metabolism failure in the cochlea, 3-nitropropionic acid was used to irreversibly inhibit the respiratory chain. Dose-response of the cochlear cells to 3-nitropropionic acid was analyzed in vitro. 3-Nitropropionic acid was administered onto the round window of guinea pigs. Cell death was identified by terminal transferase labeling the free 3'OH breaks in the DNA strands in vivo and propidium iodide nuclear permeation in vitro. As a result, 23.6 and 96.3 % cell death were induced by 10 and 100 mM 3-nitropropionic acid, respectively, in vitro. In the guinea pigs, 500 mM 3-nitropropionic acid induced vestibular dysfunction and severe to profound hearing losses. The cells that are the most sensitive to 3-nitropropionic acid treatment include the stria marginal and intermediate cells, epithelial cells of the Reissner's membrane, and spiral ligament fibrocytes (types II and V). Moderate sensitive cells were satellite fibrocytes of the spiral limbic central zone, osteocytes of the cochlear shell, hair cells, and spiral ganglion cells. Reduction of neurofilament in the soma and periphery processes of spiral ganglion cells occurred after the exposure. These results may be relevant to the mechanisms of injury in sudden onset sensorineural hearing loss and hazardous substance exposure-induced hearing loss.

14 Article Functional mutation of SMAC/DIABLO, encoding a mitochondrial proapoptotic protein, causes human progressive hearing loss DFNA64. 2011

Cheng, Jing / Zhu, Yuhua / He, Sudan / Lu, Yanping / Chen, Jing / Han, Bing / Petrillo, Marco / Wrzeszczynski, Kazimierz O / Yang, Shiming / Dai, Pu / Zhai, Suoqiang / Han, Dongyi / Zhang, Michael Q / Li, Wei / Liu, Xuezhong / Li, Huawei / Chen, Zheng-Yi / Yuan, Huijun. ·Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, China. ·Am J Hum Genet · Pubmed #21722859.

ABSTRACT: SMAC/DIABLO is a mitochondrial proapoptotic protein that is released from mitochondria during apoptosis and counters the inhibitory activities of inhibitor of apoptosis proteins, IAPs. By linkage analysis and candidate screening, we identified a heterozygous SMAC/DIABLO mutation, c.377C>T (p.Ser126Leu, refers to p.Ser71Leu in the mature protein) in a six-generation Chinese kindred characterized by dominant progressive nonsyndromic hearing loss, designated as DFNA64. SMAC/DIABLO is highly expressed in human embryonic ears and is enriched in the developing mouse inner-ear hair cells, suggesting it has a role in the development and homeostasis of hair cells. We used a functional study to demonstrate that the SMAC/DIABLO(S71L) mutant, while retaining the proapoptotic function, triggers significant degradation of both wild-type and mutant SMAC/DIABLO and renders host mitochondria susceptible to calcium-induced loss of the membrane potential. Our work identifies DFNA64 as the human genetic disorder associated with SMAC/DIABLO malfunction and suggests that mutant SMAC/DIABLO(S71L) might cause mitochondrial dysfunction.

15 Article Protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion. 2011

Zhai, S-Q / Guo, W / Hu, Y-Y / Yu, N / Chen, Q / Wang, J-Z / Fan, M / Yang, W-Y. ·Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China. gaochunyan1954@163.com ·J Laryngol Otol · Pubmed #21078216.

ABSTRACT: OBJECTIVE: To explore the protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion. METHODS: Recombinant adenovirus brain-derived neurotrophic factor vector, recombinant adenovirus LacZ and artificial perilymph were prepared. Guinea pigs with audiometric auditory brainstem response thresholds of more than 75 dB SPL, measured seven days after four hours of noise exposure at 135 dB SPL, were divided into three groups. Adenovirus brain-derived neurotrophic factor vector, adenovirus LacZ and perilymph were infused into the cochleae of the three groups, variously. Eight weeks later, the cochleae were stained immunohistochemically and the spiral ganglion cells counted. RESULTS: The auditory brainstem response threshold recorded before and seven days after noise exposure did not differ significantly between the three groups. However, eight weeks after cochlear perfusion, the group receiving brain-derived neurotrophic factor had a significantly decreased auditory brainstem response threshold and increased spiral ganglion cell count, compared with the adenovirus LacZ and perilymph groups. CONCLUSION: When administered via cochlear infusion following noise damage, brain-derived neurotrophic factor appears to improve the auditory threshold, and to have a protective effect on the spiral ganglion cells.

16 Article [Preliminary clinical research of cochlear implantation in elderly and pre-elderly patients with profound hearing loss]. 2010

Yang, Shi-ming / Li, Jia-nan / Ji, Fei / Chen, Ai-ting / Hong, Meng-di / Dai, Pu / Xi, Xin / Han, Dong-yi / Zhai, Suo-qiang. ·Otorhinolaryngology Head and Neck Surgery Hospital, General Hospital of Chinese People's Liberation Army, Beijing 100853, China. yangsm301@263.net ·Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi · Pubmed #21176571.

ABSTRACT: OBJECTIVE: To explore the safety and efficacy of cochlear implantation among elderly patients with severe to profound hearing loss. METHODS: Eight pre-elderly and elderly patients with an medium age of 58 years who suffered from bilateral severe to profound sensorineural hearing loss received cochlear implantation between November 2008 and November 2009. The patients' tolerance to implant surgery and the occurrence of complications were observed. Three months after switch-on, aided threshold and speech performance were measured. RESULTS: The surgery was uneventful in all cases with normal intraoperative neural response telemetry elicited. Three months after switch-on, average aided threshold across speech frequencies was 35 - 50 dB HL measured in sound field with warble tone. The results of speech audiometry showed large variation between individuals. Some patients achieved good performance in monosyllable recognition test, disyllables threshold test and sentences recognition test under both bubble noise and quiet conditions. CONCLUSIONS: Pre-elderly and elderly patients can endure a state of general anesthesia for cochlear surgery without complications. Cochlear implant can provide reconstruction of speech recognition capabilities for elderly patients suffering from severe to profound hearing loss. Cochlear implantation can improve the quality of life of elderly patients with hearing loss.

17 Article [Study of aural rehabilitation in post-lingual deafened patients with multi-channel cochlear implant]. 2010

Li, Jianan / Xi, Xin / Hong, Mengdi / Yang, Shiming / Han, Dongyi / Zhai, Suoqiang. ·Nankai University School of Medicine, Tianjin, China. ·Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi · Pubmed #20842943.

ABSTRACT: OBJECTIVE: To seek a convenient and effective method through subjective psychophysical measurements and CAP/SIR assessment. To compare the discrimination of rehabilitation between post-lingual and pre-lingual deafened patients. METHOD: Thirty-one post-lingual cochlear implantees and 59 pre-lingual cochlear implantees, the warble tone and CAP/SIR were assessed. The discrimination of threshold levels, comfortable levels and dynamic range between post-lingual and pre-lingual deafened patients in same electrodes were compared. RESULT: There was no statistic difference in warble tone, T-levels, C-levels and dynamic range in same electrodes implant after 6 months implant (P>0.05). The score of CAP and SIR in post-lingual deafened patients were more prominent. CONCLUSION: CAP and SIR is a kind of convenient and effective method to assess the ability of aural and oral. There was no discrimination in warble tone, T-levels, C-levels and dynamic range in same electrodes implant, but the ability of aural and oral in post-lingual deafened patients were more prominent.